Effectiveness Of Moringa Leaf Extract (Moringa oleifera) on Cadmium and LDL (Low-Density Lipoprotein) Cholesterol Levels in Blood as Indicators of Atherosclerosis in Cadmium (Cd) Induced White Rats (Rattus norvegicus) With Atomic Absorption Spectrophotometry Method (AAS)

Nabilla, Vernanda Arsya and Lestari, Indah and Rahayuningsih, Christ Kartika and Christyaningsih, Juliana (2022) Effectiveness Of Moringa Leaf Extract (Moringa oleifera) on Cadmium and LDL (Low-Density Lipoprotein) Cholesterol Levels in Blood as Indicators of Atherosclerosis in Cadmium (Cd) Induced White Rats (Rattus norvegicus) With Atomic Absorption Spectrophotometry Method (AAS). Jurnal Teknokes, 15 (3). pp. 128-136. ISSN 2407-896

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Abstract

Moringa leaves (Moringa oleifera) have chelating agent properties that act as natural antioxidants in binding heavy metals and free radical scavengers, with cadmium being one of the heavy metals free radicals having toxic effects on the body. Exposure to cadmium can reduce the activity of lipoprotein lipase enzymes (LPL) which function in the triglycerides and free fatty acids catabolism process, and can increase cholesterol and triglyceride levels in the blood. This study aimed to determine the effectiveness of Moringa (Moringa oleifera) on Cadmium chloride-induced white rats (Rattus norvegicus) using an experimental research design with a quantitative analysis technique. All authors contributed and discussed the results and final draft of the manuscript. This study used the level of cadmium and LDL cholesterol for the dependent variable and the dose of Moringa leaf extract (Moringa oleifera) for its independent variable. The samples used were white rats (Rattus norvegicus) aged 8-12 weeks with a weight of 150-200 grams, along with blood drawn from the heart for the specimen. The researcher conducted the study at the Faculty of Veterinary Medicine, Airlangga University; Surabaya Health Laboratory Center; and Surabaya Bakti Analysis Laboratory from October 2021 to June 2022 using Atomic Absorption Spectrophotometry (AAS) and Chemical Analyzer BS-200. The results showed that the average value of cadmium and LDL cholesterol levels in white rats in treatment group 1 were 0.605 g/dL and 8.25 mg/dL, group 2 with 0.075 g/dL & 18.5 mg/dL; and group 3 were 0.08125 g/dL and 12 mg/dL. Based on the statistical analysis of the One-Way ANOVA test, the sig. p-value was > 0.05. Thus, the average value of cadmium and LDL cholesterol had no significant difference. Moringa leaf extract dose 600 mg/kgBW has good effectiveness as a chelating agent that can reduce LDL cholesterol due to exposure to heavy metal cadmium in Rattus norvegicus. This study recommends consuming fresh Moringa leaves (Moringa oleifera) as much as 66.522 grams per human/day as an additional antioxidant and chelating agent. In future research, moringa oleifera fresh leaves can be used as medicine according to the dose.

Item Type:	Article
Uncontrolled Keywords:	Atomic Absorption Spectrophotometry (AAS),Cadmium,Chemical Analyzer,LDL Cholesterol,Moringa oleifera,White Rats
Subjects:	Q Science > QR Microbiology
Divisions:	Poltekkes Kemenkes Surabaya > Pusat Penelitan dan Pengabdian Masyarakat > Penelitian
Depositing User:	Noer Amelia
Date Deposited:	17 Apr 2023 04:37
Last Modified:	17 Apr 2023 04:37
URI:	http://repo.poltekkesdepkes-sby.ac.id/id/eprint/6782

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