Deteksi Gen Penyandi Resistensi Insektisida Karbamat (ace-1) pada Nyamuk Aedes Aegypti Metode PCR

MAFTUKHAH, REVI ZAKIYATUL and Sasongkowati, Retno and Istanto, Wisnu and Anggraini, Anita Dwi (2022) Deteksi Gen Penyandi Resistensi Insektisida Karbamat (ace-1) pada Nyamuk Aedes Aegypti Metode PCR. Malahayati Nursing Journal, 4 (10). pp. 2573-2583. ISSN 2655-2728

Text SEKTISIDA_KARBAMAT_ACE-1_PADA_NYAMUK_AEDES_AEGYPTIMETODE_PCR.pdf.pdf Download (2MB)

Abstract

Dengue Hemorrhagic Fever (DHF) is a high endemic problems, especially in the tropics. Effort to prepare for the surge in cases, it is necessary to develop in molecular level, detection of the Ace-1 gene can be used as an important indication of the resistance Aedes aegypti to carbamate insecticides. Purpose to detect the presence or absence of the Ace-1 gene of Aedes aegypti against carbamate insecticides using the PCR method. Methods descriptive quantitative analytic method of observational data. Treatments in experiment consisted of four test bottles and one control bottle. The results that appear on the detection of Ace-1 gene using RT-PCR are CT values. Results showed that from 4 existing samples, 2 of them were positive with a value, sample 1 (A01) had a CT value of 1.00, sample 4 (A04) had a CT value of 4.42 while other 2 were negative which was indicated by the emergence of N/A which means Not Available or not available primary gene in target gene. Conclusion percentage obtained was 50% of samples with Ace-1 gene detected and 50% of other samples not detecting Ace-1 gene.

Item Type:	Article
Uncontrolled Keywords:	Aedes aegypti, Ace-1, CT value
Subjects:	Q Science > QR Microbiology
Divisions:	Poltekkes Kemenkes Surabaya > Pusat Penelitan dan Pengabdian Masyarakat > Penelitian
Depositing User:	Noer Amelia
Date Deposited:	12 Apr 2023 03:14
Last Modified:	12 Apr 2023 03:14
URI:	http://repo.poltekkesdepkes-sby.ac.id/id/eprint/6668

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