DEWI, EKA SUKMAWATI (2022) DETEKSI GEN SHV PADA Escherichia coli PENGHASIL Extended Spectrum Beta Lactamases (ESBLs) DARI SAMPEL URIN PASIEN PENDERITA INFEKSI SALURAN KEMIH (ISK). Skripsi thesis, POLTEKKES KEMENKES SURABAYA.
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Abstract
Urinary tract infection (UTI) is one of the infectious diseases that are often found in the community. UTI is a condition in which there are microorganisms in the urinary tract characterized by bacterial colonization in the urinary tract. Escherichia coli becomes one of the most frequent causes of infecting the urinary tract. This is due to E. coli has virulence factors that can increase the colonization and invasion of bacteria into the urinary tract to cause infection. Escherichia coli is known to be able to produce Extended Spectrum Beta-Lactamases (ESBLS) which can result in producing bacteria that are resistant to beta lactam antibiotics. ESBLS is encoded by several genes, one of which is the Sulfhydryl Variable (SHV) gene. The purpose of this study was to detect the presence of SHV gene in Escherichia coli producing Extended Spectrum Beta Lactamases (ESBLS) from urine samples of patients with urinary tract infection (UTI) using Polymerase Chain Reaction (PCR) method. This research was conducted in April-May 2022 at the Bacteriology Laboratory of Poltekkes Surabaya Department of Medical Laboratory Technology and the Surabaya Health Laboratory Center. UTI urine sample obtained from RSPAL Dr. Ramelan Surabaya. This type of research is Descriptive Laboratory. The results of this study from 30 urine samples, 14 of which were identified as Escherichia coli (46%), ESBLS screening test obtained 2 samples of S3 and S24 codes resistant to ceftriaxone and cefataxime antibiotics, ESBLS confirmation test using Double Disck Synergy Test (DDST) method was obtained by S3 code samples to produce ESBLS. PCR test results of S3 code sample negative SHV gene. Keywords: UTI, E.coli, ESBLS, SHV gene.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Contributors: | Contribution Contributor NIDN/NIDK UNSPECIFIED SULIATI, SULIATI 4005096401 UNSPECIFIED ANGGRAINI, ANITA DWI UNSPECIFIED |
| Additional Information: | 12SKRAJ2022071 |
| Uncontrolled Keywords: | UTI, E.coli, ESBLS, SHV gene. |
| Subjects: | Q Science > QR Microbiology |
| Divisions: | Poltekkes Kemenkes Surabaya > Jurusan Analis Kesehatan > Program Studi Sarjana Terapan Teknologi Laboratorium Medis |
| Depositing User: | Noer Amelia |
| Date Deposited: | 09 Sep 2022 09:38 |
| Last Modified: | 07 Mar 2023 02:55 |
| URI: | http://repo.poltekkesdepkes-sby.ac.id/id/eprint/5662 |
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